Effect of Culture at Low Oxygen Tension on the Expression of Heat Shock Proteins in a Panel of Melanoma Cell LinesReportar como inadecuado




Effect of Culture at Low Oxygen Tension on the Expression of Heat Shock Proteins in a Panel of Melanoma Cell Lines - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Objective

Tumours are commonly hypoxic and this can be associated with aggressive tumour type, metastasis and resistance to therapy. Heat shock proteins hsps are induced in response to hypoxia, provide cancer cells with protection against tumour-associated stressors and chaperone oncoproteins that drive tumour proliferation. This study examined the effect of different oxygen concentrations on the expression of hsps in melanoma cell lines.

Methods

Melanoma cell lines were cultured in 2% and 20% O2. Expression of Hsp90, Hsp70, Hsp60, Hsp40 and Hsp32 proteins were determined by flow cytometry.

Results

Growth rates and viability were reduced in the majority of cell lines by culture in 2% O2. Hsp expression was different in 2% compared to 20% O2 and changes in Hsp90 expression correlated with cell line generation time P<0.005 and viability P<0.01. Greater total hsp expression correlated with improved viability in 2% but not 20% O2 P<0.05. Relative expression of the different hsps was consistent across cell lines and each correlated with the others P = 0.0001 but not with Hsp32. Hsp expression was inversely correlated with cell line adhesion to laminin as well as collagen type IV and Breslow depth of the original primary tumour tissue P<0.05, but not with Clark level or patient survival. All five hsps were identified on the cell surface.

Conclusion

Culture in 2% O2 variably altered hsp expression in a panel of melanoma cell lines. Hsp expression was associated with certain cell line characteristics and clinical parameters of the originating tumour.



Autor: Christopher Shipp , Evelyna Derhovanessian, Graham Pawelec

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados