Gephyrin-Independent GABAAR Mobility and Clustering during PlasticityReportar como inadecuado

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The activity-dependent modulation of GABA-A receptor GABAAR clustering at synapses controls inhibitory synaptic transmission. Several lines of evidence suggest that gephyrin, an inhibitory synaptic scaffold protein, is a critical factor in the regulation of GABAAR clustering during inhibitory synaptic plasticity induced by neuronal excitation. In this study, we tested this hypothesis by studying relative gephyrin dynamics and GABAAR declustering during excitatory activity. Surprisingly, we found that gephyrin dispersal is not essential for GABAAR declustering during excitatory activity. In cultured hippocampal neurons, quantitative immunocytochemistry showed that the dispersal of synaptic GABAARs accompanied with neuronal excitation evoked by 4-aminopyridine 4AP or N-methyl-D-aspartic acid NMDA precedes that of gephyrin. Single-particle tracking of quantum dot labeled-GABAARs revealed that excitation-induced enhancement of GABAAR lateral mobility also occurred before the shrinkage of gephyrin clusters. Physical inhibition of GABAAR lateral diffusion on the cell surface and inhibition of a Ca2+ dependent phosphatase, calcineurin, completely eliminated the 4AP-induced decrease in gephyrin cluster size, but not the NMDA-induced decrease in cluster size, suggesting the existence of two different mechanisms of gephyrin declustering during activity-dependent plasticity, a GABAAR-dependent regulatory mechanism and a GABAAR-independent one. Our results also indicate that GABAAR mobility and clustering after sustained excitatory activity is independent of gephyrin.

Autor: Fumihiro Niwa, Hiroko Bannai , Misa Arizono, Kazumi Fukatsu, Antoine Triller, Katsuhiko Mikoshiba



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