GABAergic Transmission and Chloride Equilibrium Potential Are Not Modulated by Pyruvate in the Developing Optic Tectum of Xenopus laevis TadpolesReportar como inadecuado




GABAergic Transmission and Chloride Equilibrium Potential Are Not Modulated by Pyruvate in the Developing Optic Tectum of Xenopus laevis Tadpoles - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

In the developing mammalian brain, gamma-aminobutyric acid GABA is thought to play an excitatory rather than an inhibitory role due to high levels of intracellular Cl− in immature neurons. This idea, however, has been questioned by recent studies which suggest that glucose-based artificial cerebrospinal fluid ACSF may be inadequate for experiments on immature and developing brains. These studies suggest that immature neurons may require alternative energy sources, such as lactate or pyruvate. Lack of these other energy sources is thought to result in artificially high intracellular Cl− concentrations, and therefore a more depolarized GABA receptor GABAR reversal potential. Since glucose metabolism can vary widely among different species, it is important to test the effects of these alternative energy sources on different experimental preparations. We tested whether pyruvate affects GABAergic transmission in isolated brains of developing wild type Xenopus tadpoles in vitro by recording the responsiveness of tectal neurons to optic nerve stimulation, and by measuring currents evoked by local GABA application in a gramicidin perforated patch configuration. We found that, in contrast with previously reported results, the reversal potential for GABAR-mediated currents does not change significantly between developmental stages 45 and 49. Partial substitution of glucose by pyruvate had only minor effects on both the GABA reversal potential, and the responsiveness of tectal neurons at stages 45 and 49. Total depletion of energy sources from the ACSF did not affect neural responsiveness. We also report a strong spatial gradient in GABA reversal potential, with immature cells adjacent to the lateral and caudal proliferative zones having more positive reversal potentials. We conclude that in this experimental preparation standard glucose-based ACSF is an appropriate extracellular media for in vitro experiments.



Autor: Arseny S. Khakhalin, Carlos D. Aizenman

Fuente: http://plos.srce.hr/



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