Macrophage Sub-Populations and the Lipoxin A4 Receptor Implicate Active Inflammation during Equine Tendon RepairReportar como inadecuado

Macrophage Sub-Populations and the Lipoxin A4 Receptor Implicate Active Inflammation during Equine Tendon Repair - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Macrophages Mφ orchestrate inflammatory and reparatory processes in injured connective tissues but their role during different phases of tendon healing is not known. We investigated the contribution of different Mφ subsets in an equine model of naturally occurring tendon injury. Post mortem tissues were harvested from normal uninjured, sub-acute 3–6 weeks post injury and chronically injured >3 months post injury superficial digital flexor tendons. To determine if inflammation was present in injured tendons, Mφ sub-populations were quantified based on surface antigen expression of CD172a pan Mφ, CD14highCD206low pro-inflammatory M1Mφ, and CD206high anti-inflammatory M2Mφ to assess potential polarised phenotypes. In addition, the Lipoxin A4 receptor FPR2-ALX was used as marker for resolving inflammation. Normal tendons were negative for both Mφ and FPR2-ALX. In contrast, M1Mφ predominated in sub-acute injury, whereas a potential phenotype-switch to M2Mφ polarity was seen in chronic injury. Furthermore, FPR2-ALX expression by tenocytes was significantly upregulated in sub-acute but not chronic injury. Expression of the FPR2-ALX ligand Annexin A1 was also significantly increased in sub-acute and chronic injuries in contrast to low level expression in normal tendons. The combination of reduced FPR2-ALX expression and persistence of the M2Mφ phenotype in chronic injury suggests a potential mechanism for incomplete resolution of inflammation after tendon injury. To investigate the effect of pro-inflammatory mediators on lipoxin A4 LXA4 production and FPR2-ALX expression in vitro, normal tendon explants were stimulated with interleukin-1 beta and prostaglandin E2. Stimulation with either mediator induced LXA4 release and maximal upregulation of FPR2-ALX expression after 72 hours. Taken together, our data suggests that although tenocytes are capable of mounting a protective mechanism to counteract inflammatory stimuli, this appears to be of insufficient duration and magnitude in natural tendon injury, which may potentiate chronic inflammation and fibrotic repair, as indicated by the presence of M2Mφ.

Autor: Stephanie Georgina Dakin , Dirk Werling, Andrew Hibbert, Dilkush Robert Ephrem Abayasekara, Natalie Jayne Young, Roger Kenneth Wh



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