Rectangular Coordination Polymer Nanoplates: Large-Scale, Rapid Synthesis and Their Application as a Fluorescent Sensing Platform for DNA DetectionReportar como inadecuado




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In this paper, we report on the large-scale, rapid synthesis of uniform rectangular coordination polymer nanoplates RCPNs assembled from CuII and 4,4′-bipyridine for the first time. We further demonstrate that such RCPNs can be used as a very effective fluorescent sensing platform for multiple DNA detection with a detection limit as low as 30 pM and a high selectivity down to single-base mismatch. The DNA detection is accomplished by the following two steps: 1 RCPN binds dye-labeled single-stranded DNA ssDNA probe, which brings dye and RCPN into close proximity, leading to fluorescence quenching; 2 Specific hybridization of the probe with its target generates a double-stranded DNA dsDNA which detaches from RCPN, leading to fluorescence recovery. It suggests that this sensing system can well discriminate complementary and mismatched DNA sequences. The exact mechanism of fluorescence quenching involved is elucidated experimentally and its use in a human blood serum system is also demonstrated successfully.



Autor: Yingwei Zhang, Yonglan Luo, Jingqi Tian, Abdullah M. Asiri, Abdulrahman O. Al-Youbi, Xuping Sun

Fuente: http://plos.srce.hr/



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