Ultrasound Biomicroscopy for Longitudinal Studies of Carotid Plaque Development in Mice: Validation with Histological EndpointsReportar como inadecuado




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Atherosclerosis is responsible for the death of thousands of Americans each year. The carotid constriction model of plaque development has recently been presented as a model for unstable plaque formation in mice. In this study we 1 validate ultrasound biomicroscopy UBM for the determination of carotid plaque size, percent stenosis, and plaque development in live animals, 2 determine the sensitivity of UBM in detecting changes in blood flow induced by carotid constriction and 3 test whether plaque formation can be predicted from blood flow parameters measured by UBM. Carotid plaques were induced by surgical constriction in Apo E−-− mice. Arteries were imaged bi-weekly by UBM, at which time PW-Doppler measurements of proximal blood flow, as well as plaque length and percent stenosis were determined. Histology was performed 9 weeks post surgery. When compared to whole mount post-mortem measurements, UBM accurately reported carotid plaque length. Percent stenosis, based on transverse B-mode UBM measurements, correlated well with that calculated from histological sections. PW-Doppler revealed that constriction reduced maximum systolic velocity vmax and duration of the systolic velocity peak ts-tt. Pre-plaque 2 week post-surgery PW-Doppler parameters vmax and ts-tt were correlated with plaque length at 9 weeks, and were predictive of plaque formation. Correlation of initiating PW-Doppler parameters vmax and ts-tt with resulting plaque length established the degree of flow disturbance required for subsequent plaque development and demonstrated its power for predicting plaque development.



Autor: Erin Y. Harmon, Van Fronhofer, Rebecca S. Keller, Paul J. Feustel, M. Julia Brosnan, Jan H. von der Thüsen, Daniel J. Loegering,

Fuente: http://plos.srce.hr/



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