Capture of Neuroepithelial-Like Stem Cells from Pluripotent Stem Cells Provides a Versatile System for In Vitro Production of Human NeuronsReportar como inadecuado




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Human embryonic stem cells hESC and induced pluripotent stem cells iPSC provide new prospects for studying human neurodevelopment and modeling neurological disease. In particular, iPSC-derived neural cells permit a direct comparison of disease-relevant molecular pathways in neurons and glia derived from patients and healthy individuals. A prerequisite for such comparative studies are robust protocols that efficiently yield standardized populations of neural cell types. Here we show that long-term self-renewing neuroepithelial-like stem cells lt-NES cells derived from 3 hESC and 6 iPSC lines in two independent laboratories exhibit consistent characteristics including i continuous expandability in the presence of FGF2 and EGF; ii stable neuronal and glial differentiation competence; iii characteristic transcription factor profile; iv hindbrain specification amenable to regional patterning; v capacity to generate functionally mature human neurons. We further show that lt-NES cells are developmentally distinct from fetal tissue-derived radial glia-like stem cells. We propose that lt-NES cells provide an interesting tool for studying human neurodevelopment and may serve as a standard system to facilitate comparative analyses of hESC and hiPSC-derived neural cells from control and diseased genetic backgrounds.



Autor: Anna Falk , Philipp Koch , Jaideep Kesavan, Yasuhiro Takashima, Julia Ladewig, Michael Alexander, Ole Wiskow, Jignesh Tailor, Mat

Fuente: http://plos.srce.hr/



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