Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from SoilReportar como inadecuado




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A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford and Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.



Autor: Kurt Showmaker, Gary W. Lawrence, Shien Lu, Clarissa Balbalian, Vincent P. Klink

Fuente: http://plos.srce.hr/



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