Comparative Microarray Analysis of Intestinal Lymphocytes following Eimeria acervulina, E. maxima, or E. tenella Infection in the ChickenReportar como inadecuado




Comparative Microarray Analysis of Intestinal Lymphocytes following Eimeria acervulina, E. maxima, or E. tenella Infection in the Chicken - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Relative expression levels of immune- and non-immune-related mRNAs in chicken intestinal intraepithelial lymphocytes experimentally infected with Eimeria acervulina, E. maxima, or E. tenella were measured using a 10K cDNA microarray. Based on a cutoff of >2.0-fold differential expression compared with uninfected controls, relatively equal numbers of transcripts were altered by the three Eimeria infections at 1, 2, and 3 days post-primary infection. By contrast, E. tenella elicited the greatest number of altered transcripts at 4, 5, and 6 days post-primary infection, and at all time points following secondary infection. When analyzed on the basis of up- or down-regulated transcript levels over the entire 6 day infection periods, approximately equal numbers of up-regulated transcripts were detected following E. tenella primary 1,469 and secondary 1,459 infections, with a greater number of down-regulated mRNAs following secondary 1,063 vs. primary 890 infection. On the contrary, relatively few mRNA were modulated following primary infection with E. acervulina 35 up, 160 down or E. maxima 65 up, 148 down compared with secondary infection E. acervulina, 1,142 up, 1,289 down; E. maxima, 368 up, 1,349 down. With all three coccidia, biological pathway analysis identified the altered transcripts as belonging to the categories of -Disease and Disorder- and -Physiological System Development and Function-. Sixteen intracellular signaling pathways were identified from the differentially expressed transcripts following Eimeria infection, with the greatest significance observed following E. acervulina infection. Taken together, this new information will expand our understanding of host-pathogen interactions in avian coccidiosis and contribute to the development of novel disease control strategies.



Autor: Duk Kyung Kim, Hyun Lillehoj , Wongi Min, Chul Hong Kim, Myeong Seon Park, Yeong Ho Hong, Erik P. Lillehoj

Fuente: http://plos.srce.hr/



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