Characterization of Brca2-Deficient Plants Excludes the Role of NHEJ and SSA in the Meiotic Chromosomal Defect PhenotypeReportar como inadecuado




Characterization of Brca2-Deficient Plants Excludes the Role of NHEJ and SSA in the Meiotic Chromosomal Defect Phenotype - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

In somatic cells, three major pathways are involved in the repair of DNA double-strand breaks DBS: Non-Homologous End Joining NHEJ, Single-Strand Annealing SSA and Homologous Recombination HR. In somatic and meiotic HR, DNA DSB are 5′ to 3′ resected, producing long 3′ single-stranded DNA extensions. Brca2 is essential to load the Rad51 recombinase onto these 3′ overhangs. The resulting nucleofilament can thus invade a homologous DNA sequence to copy and restore the original genetic information. In Arabidopsis, the inactivation of Brca2 specifically during meiosis by an RNAi approach results in aberrant chromosome aggregates, chromosomal fragmentation and missegregation leading to a sterility phenotype. We had previously suggested that such chromosomal behaviour could be due to NHEJ. In this study, we show that knock-out plants affected in both BRCA2 genes show the same meiotic phenotype as the RNAi-inactivated plants. Moreover, it is demonstrated that during meiosis, neither NHEJ nor SSA compensate for HR deficiency in BRCA2-inactivated plants. The role of the plant-specific DNA Ligase6 is also excluded. The possible mechanisms involved in the formation of these aberrant chromosomal bridges in the absence of HR during meiosis are discussed.



Autor: Marilyn Dumont, Sophie Massot, Marie-Pascale Doutriaux, Ariane Gratias

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados