Reduced Expression of Transcription Factor AP-2α Is Associated with Gastric Adenocarcinoma PrognosisReportar como inadecuado




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Background

This study aims to investigate the expression and prognostic significance of activator protein 2α AP-2α in gastric adenocarcinoma.

Methodology-Principal Findings

AP-2α expression was analyzed using real-time quantitative PCR RT-qPCR, western blotting, and immunohistochemical staining methods on tissue samples from a consecutive series of 481 gastric adenocarcinoma patients who underwent resections between 2003 and 2006. The relationship between AP-2α expression, clinicopathological factors, and patient survival was investigated. RT- qPCR results showed that the expression of AP-2α mRNA was reduced in tumor tissue samples, compared with expression in matched adjacent non-tumor tissue samples P = 0.009; this finding was confirmed by western blotting analysis P = 0.012. Immunohistochemical staining data indicated that AP-2α expression was significantly decreased in 196 of 481 40.7% gastric adenocarcinoma cases; reduced AP-2α expression was also observed in patients with poorly differentiated tumors P = 0.001 and total gastric carcinomas P = 0.002, as well as in patients who underwent palliative tumor resection P = 0.004. Additionally, reduced expression of AP-2α was more commonly observed in tumors that were staged as T4a-b P = 0.018, N3 P = 0.006, and M1 P = 0.008. Kaplan-Meier survival curves revealed that reduced expression of AP-2α was associated with poor prognosis in gastric adenocarcinoma patients P<0.001. Multivariate Cox analysis identified AP-2α expression as an independent prognostic factor for overall survival HR = 1.512, 95% CI = 1.127–2.029, P = 0.006.

Conclusions-Significance

Our data suggest that AP-2α plays an important role in tumor progression and that reduced AP-2α expression independently predicts an unfavorable prognosis in gastric adenocarcinoma patients.



Autor: Wei Wang , Lin Lv , Ke Pan, Yu Zhang, Jing-jing Zhao, Ju-gao Chen, Yi-bing Chen, Yong-qiang Li, Qi-jin Wang, Jia He, Shi-ping Che

Fuente: http://plos.srce.hr/



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