Evolutionary Relationship of Wheat Protein Disulphide Isomerase PDI Gene Promoter Sequence Based on Phylogenetic AnalysisReportar como inadecuado




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Protein disulphide isomerase PDI is an oxidoreductase enzyme abundant in the endoplasmic reticulum ER. In plants, PDIs have been shown to assist the folding and deposition of seed storage proteins during the biogenesis of protein bodies in the endosperm. Cloning and characterization of the complete set of genes encoding PDI and PDI like proteins in bread wheat Triticum aestivum cv. Chinese Spring and the comparison of their sequence, structure and expression with homologous genes from other plant species were reported in our previous publications. Promoter sequences of three homoeologous genes encoding typical PDI, located on chromosome group 4 of bread wheat, and PDI promoter sequence analysis of Triticum urartu, Aegilops speltoides and Aegilops tauschii had also been reported previously. In this study, we report the isolation and sequencing of a ~700 bp region, comprising ~600 bp of the putative promoter region and 88 bp of the first exon of the typical PDI gene, in five accessions each from Triticum urartu AA, Aegilops speltoides BB and Aegilops tauschii DD. Sequence analysis indicated large variation among sequences belonging to the different genomes, while close similarity was found within each species and with the corresponding homoeologous PDI sequences of Triticum aestivum cv. CS AABBDD resulting in an overall high conservation of the sequence conferring endosperm-specific expression.

KEYWORDS

Protein Disulfide Isomerase PDI Promoter; Wheat; Phylogenetic Analysis

Cite this paper

A. Prabhu Dhanapal -Evolutionary Relationship of Wheat Protein Disulphide Isomerase PDI Gene Promoter Sequence Based on Phylogenetic Analysis,- American Journal of Plant Sciences, Vol. 3 No. 3, 2012, pp. 373-380. doi: 10.4236-ajps.2012.33045.





Autor: Arun Prabhu Dhanapal

Fuente: http://www.scirp.org/



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