Alkaline comet assay as a biomarker of DNA-damage encountered in workers engaged in cigarette manufacturingReportar como inadecuado




Alkaline comet assay as a biomarker of DNA-damage encountered in workers engaged in cigarette manufacturing - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Periodicum biologorum, Vol.111 No.1 March 2009. -

Background and Purpose: The aim of this pilot study was to determine

possible genotoxic effects of occupational exposure to tobacco dust between workers employed in tobbaco industry and non-exposed population.

Subjects and Method: The exposed group consisted of 20 subjects with mena age of 43.4 years. The average duration of their occupational exposure was 19.95 years. The control group consisted of 40 subjects, mean age 40.18 years and was choosen from general Croatian population. The assessment of primaryDNAdamage hosted by peripheral blood leukocytes was performed using the alkaline comet assay, the tail length and long-tailed nuclei thereby

being the primary outcome of the measure.

Results and Conclusions: The results showed that group comet tail

length mean measured in the exposed workers was 14.39±1.02 mm whereas mean percentage of long-tailed nuclei was 8.20. In the control group, the mean tail length was 13.91±0.66 mm and long-tailed nucleus percentage was 1.88. Mean values for the tail length measured and the percentage of long-tailed nuclei were significantly higher in the exposed group compared to the control one. Within the exposed population, significant inter-individual differences inDNAdamage were found P



Autor: Vera Garaj-Vrhovac - ; Mutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000 Zagreb,

Fuente: http://hrcak.srce.hr/



DESCARGAR PDF




Documentos relacionados