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DNA binding with one finger DOF transcription factors play important roles in storage material accumulation and morphogenesis of developing seeds. Oil and protein contents varied in different cultivars in important oil crop peanut. DOF proteins have not been studied in this crop. In this paper, we analyzed all the DOF genes expressed in developing seeds from a cDNA library with 20,000 transcripts, cloned and compared similar genes of GW391729 from eight peanut cultivars, and analyzed similar genes expressed in root and leave with control and inoculated with Ralstonia solanacearum. The results indicate that total eight types of DOF genes were expressed in developing seeds of cultivar 063103. Most of DOF transcription factors expressed involved in developmental process in a complicated way. Among them, GW391729 is possible related to the seed number in fruit, and also is possible related to leafspot resistance. Detailed function of these DOF proteins need to be further studied.

KEYWORDS

DOF; Leafspot; Peanut; Seed Development; Seed Number

Cite this paper

Yan, H. , Huang, J. , Liao, B. , Lan, X. , Luo, Q. and Tang, J. 2012 DOF transcription factors in developing peanut Arachis hypogaea seeds. American Journal of Molecular Biology, 2, 60-71. doi: 10.4236-ajmb.2012.21007.





Autor: Haiyan Yan, Jiaquan Huang, Boshou Liao, Xianqing Lan, Qiuting Luo, Junlong Tang

Fuente: http://www.scirp.org/



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