Mitotic Spindle Proteomics in Chinese Hamster Ovary CellsReportar como inadecuado

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Mitosis is a fundamental process in the development of all organisms. The mitotic spindle guides the cell through mitosis as it mediates the segregation of chromosomes, the orientation of the cleavage furrow, and the progression of cell division. Birth defects and tissue-specific cancers often result from abnormalities in mitotic events. Here, we report a proteomic study of the mitotic spindle from Chinese Hamster Ovary CHO cells. Four different isolations of metaphase spindles were subjected to Multi-dimensional Protein Identification Technology MudPIT analysis and tandem mass spectrometry. We identified 1155 proteins and used Gene Ontology GO analysis to categorize proteins into cellular component groups. We then compared our data to the previously published CHO midbody proteome and identified proteins that are unique to the CHO spindle. Our data represent the first mitotic spindle proteome in CHO cells, which augments the list of mitotic spindle components from mammalian cells.

Autor: Mary Kate Bonner, Daniel S. Poole, Tao Xu, Ali Sarkeshik, John R. Yates III, Ahna R. Skop



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