Phenotypic Characterization of HIV-Specific CD8 T Cells during Early and Chronic Infant HIV-1 InfectionReport as inadecuate




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Although CD8+ T cells play an important role in the containment of adult HIV-1 replication, their role in infant HIV-1 infection is not as well understood. Impaired HIV-specific CD8+ T cell responses may underlie the persistently high viral loads observed in infants. We examined the frequency and phenotype of infant HIV-specific CD8+ T cells in 7 HIV-infected antiretroviral therapy-naïve infants during the first 2 years of life, using class I HLA tetramers and IFN-γ-ELISPOT. The frequency 0.088–3.9% of CD3+CD8+ cells and phenotype CD27+CD28−, CD45RA+-−, CD57+-−, HLA-DR+, CD95+ of infant HIV-specific CD8+ T cells were similar to reports in adults undergoing early infection. Unlike adults, at 23–24 months post-infection a high frequency of HIV-specific CD8+ T cells expressed HLA-DR mean 80%, range 68–85% and CD95 mean 88%, range 79–96%, suggesting sustained activation and vulnerability to apoptosis. Despite comparable expansion of HIV-specific CD8+ T cells of a similar phenotype to adults during early infection, infant T cells failed to contain HIV-1 replication, and remained persistently activated and vulnerable to apoptosis during chronic infection.



Author: Jennifer A. Slyker , Grace C. John-Stewart, Tao Dong, Barbara Lohman-Payne, Marie Reilly, Ann Atzberger, Stephen Taylor, Elizabet

Source: http://plos.srce.hr/



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