Role of Ox-PAPCs in the Differentiation of Mesenchymal Stem Cells MSCs and Runx2 and PPARγ2 Expression in MSCs-Like of Osteoporotic PatientsReport as inadecuate




Role of Ox-PAPCs in the Differentiation of Mesenchymal Stem Cells MSCs and Runx2 and PPARγ2 Expression in MSCs-Like of Osteoporotic Patients - Download this document for free, or read online. Document in PDF available to download.

Background

Mesenchymal stem cells MSCs can differentiate into osteoblasts and adipocytes and conditions causing bone loss may induce a switch from the osteoblast to adipocyte lineage. In addition, the expression of Runx2 and the PPARγ2 transcription factor genes is essential for cellular commitment to an osteogenic and adipogenic differentiation, respectively.Modified lipoproteins derived from the oxidation of arachidonate-containing phospholipids ox-PAPCs: POVPC, PGPC and PEIPC are considered important factors in atherogenesis.

Methodology

We investigated the effect of ox-PAPCs on osteogenesis and adipogenesis in human mesenchymal stem cells hMSCs. In particular, we analyzed the transcription factor Runx2 and the PPARγ2 gene expression during osteogenic and adipogenic differentiation in absence and in presence of ox-PAPCs. We also analyzed gene expression level in a panel of osteoblastic and adipogenic differentiation markers.In addition, as circulating blood cells can be used as a -sentinel- that responds to changes in the macro- or micro-environment, we analyzed the Runx2 and the PPARγ2 gene expression in MSCs-like and ox-PAPC levels in serum of osteoporotic patients OPs. Finally, we examined the effects of sera obtained from OPs in hMSCs comparing the results with age-matched normal donors NDs.

Principal findings

Quantitative RT-PCR demonstrated that ox-PAPCs enhanced PPARγ2 and adipogenic gene expression and reduced Runx2 and osteoblast differentiation marker gene expression in differentiating hMSCs. In OPs, ox-PAPC levels and PPARγ2 expression were higher than in NDs, whereas Runx2 was lower than in ND circulant MSCs-like.

Conclusions

Ox-PAPCs affect the osteogenic differentiation by promoting adipogenic differentiation and this effect may appear involved in bone loss in OPs.



Author: Maria Teresa Valenti, Ulisse Garbin, Andrea Pasini, Mirko Zanatta, Chiara Stranieri, Stefania Manfro, Chiara Zucal, Luca Dalle Ca

Source: http://plos.srce.hr/



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