Visfatin Is Regulated by Rosiglitazone in Type 2 Diabetes Mellitus and Influenced by NFκB and JNK in Human Abdominal Subcutaneous AdipocytesReport as inadecuate




Visfatin Is Regulated by Rosiglitazone in Type 2 Diabetes Mellitus and Influenced by NFκB and JNK in Human Abdominal Subcutaneous Adipocytes - Download this document for free, or read online. Document in PDF available to download.

Visfatin has been proposed as an insulin-mimicking adipocytokine, predominantly secreted from adipose tissue and correlated with obesity. However, recent studies suggest visfatin may act as a proinflammatory cytokine. Our studies sought to determine the significance of this adipocytokine and its potential role in the pathogenesis of T2DM. Firstly, we examined the effects of diabetic status on circulating visfatin levels, and several other adipocytokines, demonstrating that diabetic status increased visfatin*, TNF-α*** and IL-6*** compared with non-diabetic subjects *p<0.05, **p<0.01, ***p<0.001, respectively. We then assessed the effects of an insulin sensitizer, rosiglitazone RSG, in treatment naïve T2DM subjects, on circulating visfatin levels. Our findings showed that visfatin was reduced post-RSG treatment vs. pre-treatment *p<0.05 accompanied by a reduction in HOMA-IR**, thus implicating a role for insulin in visfatin regulation. Further studies addressed the intracellular mechanisms by which visfatin may be regulated, and may exert pro-inflammatory effects, in human abdominal subcutaneous Abd Sc adipocytes. Following insulin Ins and RSG treatment, our in vitro findings highlighted that insulin 100 nM, alone, upregulated visfatin protein expression whereas, in combination with RSG 10 nM, it reduced visfatin*, IKKβ** and p-JNK1-2*. Furthermore, inhibition of JNK protein exacted a significant reduction in visfatin expression **p<0.01, whilst NF-κB blockade increased visfatin *p<0.05, thus identifying JNK as the more influential factor in visfatin regulation. Additional in vitro analysis on adipokines regulating visfatin showed that only Abd Sc adipocytes treated with recombinant human rhIL-6 increased visfatin protein *p<0.05, whilst rh visfatin treatment, itself, had no influence on TNF-α, IL-6 or resistin secretion from Sc adipocytes. These data highlight visfatin-s regulation by insulin and RSG, potentially acting through NF-κB and JNK mechanisms, with only rh IL-6 modestly affecting visfatin regulation. Taken together, these findings suggest that visfatin may represent a pro-inflammatory cytokine that is influenced by insulin-insulin sensitivity via the NF-κB and JNK pathways.



Author: Kirsty C. McGee , Alison L. Harte , Nancy F. da Silva, Nasser Al-Daghri, Steven J. Creely, Christine M. Kusminski, Gyanendra Trip

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents