In Situ Prior Proliferation of CD4 CCR6 Regulatory T Cells Facilitated by TGF-β Secreting DCs Is Crucial for Their Enrichment and Suppression in Tumor ImmunityReport as inadecuate




In Situ Prior Proliferation of CD4 CCR6 Regulatory T Cells Facilitated by TGF-β Secreting DCs Is Crucial for Their Enrichment and Suppression in Tumor Immunity - Download this document for free, or read online. Document in PDF available to download.

Background

CD4+CD25+ regulatory T cells Tregs, a heterogeneous population, were enrichment in tumor mass and played an important role in modulating anti-tumor immunity. Recently, we reported a Treg subset, CCR6+ Tregs but not CCR6−Tregs, were enriched in tumor mass and closely related to poor prognosis of breast cancer patients. However, the underlying mechanism remains elusive. Here, we carefully evaluate the enrichment of CCR6+Tregs in tumor mass during progression of breast cancer and explore its possible mechanism.

Methodology-Principal Findings

The frequency of CCR6+Tregs in tumor infiltrating lymphocytes TILs was analyzed at early stage and at late stage of tumor in a murine breast cancer model by FACS respectively. The expansion of CCR6+Tregs and their CCR6− counterpart in tumor mass were determined by BrdU incorporation assay. The effect and its possible mechanism of tumor-resident antigen presenting cells APCs on the proliferation of CCR6+Tregs also were evaluated. The role of local expansion of CCR6+Tregs in their enrichment and suppression in vivo also was evaluated in adoptive cell transfer assay. We found that the prior enrichment of CCR6+Tregs but not CCR6−Tregs in tumor mass during progression of murine breast cancer, which was dependent on the dominant proliferation of CCR6+ Tregs in situ. Further study demonstrated that tumor-resident DCs triggered the proliferation of CCR6+Treg cells in TGF-β dependent manner. Adoptive transfer of CCR6+Tregs was found to potently inhibit the function of CD8+T cells in vivo, which was dependent on their proliferation and subsequently enrichment in tummor mass.

Conclusions-Significance

Our finding suggested that CCR6+ Tregs, a distinct subset of Tregs, exert its predominant suppressive role in tumor immunity through prior in situ expansion, which might ultimately provide helpful thoughts for the designing of Treg-based immunotherapy for tumor in the future.



Author: Lin Xu, Wei Xu, Zhenke Wen, Sidong Xiong

Source: http://plos.srce.hr/



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