Characterization of Novel StAR Steroidogenic Acute Regulatory Protein Mutations Causing Non-Classic Lipoid Adrenal HyperplasiaReport as inadecuate




Characterization of Novel StAR Steroidogenic Acute Regulatory Protein Mutations Causing Non-Classic Lipoid Adrenal Hyperplasia - Download this document for free, or read online. Document in PDF available to download.

Context

Steroidogenic acute regulatory protein StAR is crucial for transport of cholesterol to mitochondria where biosynthesis of steroids is initiated. Loss of StAR function causes lipoid congenital adrenal hyperplasia LCAH.

Objective

StAR gene mutations causing partial loss of function manifest atypical and may be mistaken as familial glucocorticoid deficiency. Only a few mutations have been reported.

Design

To report clinical, biochemical, genetic, protein structure and functional data on two novel StAR mutations, and to compare them with published literature.

Setting

Collaboration between the University Children-s Hospital Bern, Switzerland, and the CIBERER, Hospital Vall d-Hebron, Autonomous University, Barcelona, Spain.

Patients

Two subjects of a non-consanguineous Caucasian family were studied. The 46,XX phenotypic normal female was diagnosed with adrenal insufficiency at the age of 10 months, had normal pubertal development and still has no signs of hypergonodatropic hypogonadism at 32 years of age. Her 46,XY brother was born with normal male external genitalia and was diagnosed with adrenal insufficiency at 14 months. Puberty was normal and no signs of hypergonadotropic hypogonadism are present at 29 years of age.

Results

StAR gene analysis revealed two novel compound heterozygote mutations T44HfsX3 and G221S. T44HfsX3 is a loss-of-function StAR mutation. G221S retains partial activity ∼30% and is therefore responsible for a milder, non-classic phenotype. G221S is located in the cholesterol binding pocket and seems to alter binding-release of cholesterol.

Conclusions

StAR mutations located in the cholesterol binding pocket V187M, R188C, R192C, G221D-S seem to cause non-classic lipoid CAH. Accuracy of genotype-phenotype prediction by in vitro testing may vary with the assays employed.



Author: Christa E. Flück , Amit V. Pandey, Bernhard Dick, Núria Camats, Mónica Fernández-Cancio, María Clemente, Miquel Gussinyé, A

Source: http://plos.srce.hr/



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