Localization of Human RNase Z Isoforms: Dual Nuclear-Mitochondrial Targeting of the ELAC2 Gene Product by Alternative Translation InitiationReportar como inadecuado




Localization of Human RNase Z Isoforms: Dual Nuclear-Mitochondrial Targeting of the ELAC2 Gene Product by Alternative Translation Initiation - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

RNase Z is an endonuclease responsible for the removal of 3′ extensions from tRNA precursors, an essential step in tRNA biogenesis. Human cells contain a long form RNase ZL encoded by ELAC2, and a short form RNase ZS; ELAC1. We studied their subcellular localization by expression of proteins fused to green fluorescent protein. RNase ZS was found in the cytosol, whereas RNase ZL localized to the nucleus and mitochondria. We show that alternative translation initiation is responsible for the dual targeting of RNase ZL. Due to the unfavorable context of the first AUG of ELAC2, translation apparently also starts from the second AUG, whereby the mitochondrial targeting sequence is lost and the protein is instead routed to the nucleus. Our data suggest that RNase ZL is the enzyme involved in both, nuclear and mitochondrial tRNA 3′ end maturation.



Autor: Walter Rossmanith

Fuente: http://plos.srce.hr/



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