Sub-Telomeric core X and Y Elements in S.cerevisiae Suppress Extreme Variations in Gene SilencingReportar como inadecuado




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Telomere Position Effect TPE is governed by strong repression signals emitted by telomeres via the Sir2-3-4 Histone Deacetylase complex. These signals are then relayed by weak proto-silencers residing in the subtelomeric core X and Y- elements. Subtelomeres also contain Sub-Telomeric Anti-silencing Regions STARs. In this study we have prepared telomeres built of different combinations of core X, Y- and STARs and have analyzed them in strains lacking Histone-Acetyltransferase genes as well as in cdc6-1 and Δrif1 strains. We show that core X and Y- dramatically reduce both positive and negative variations in TPE, that are caused by these mutations. We also show that the deletion of Histone-Acetyltransferase genes reduce the silencing activity of an ACS proto-silencer, but also reduce the anti-silencing activity of a STAR. We postulate that core X and Y- act as epigenetic -cushioning- cis-elements.



Autor: Patricia Power , Daniel Jeffery , Muhammad Attiq Rehman , Arjun Chatterji, Krassimir Yankulov

Fuente: http://plos.srce.hr/



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