A Plant-Specific Transcription Factor IIB-Related Protein, pBRP2, Is Involved in Endosperm Growth ControlReportar como inadecuado




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General transcription factor IIB TFIIB and TFIIB-related factor BRF, are conserved RNA polymerase II-III RNAPII-III selectivity factors that are involved in polymerase recruitment and transcription initiation in eukaryotes. Recent findings have shown that plants have evolved a third type of B-factor, plant-specific TFIIB-related protein 1 pBRP1, which seems to be involved in RNAPI transcription. Here, we extend the repertoire of B-factors in plants by reporting the characterization of a novel TFIIB-related protein, plant-specific TFIIB-related protein 2 pBRP2, which is found to date only in the Brassicacea family. Unlike other B-factors that are ubiquitously expressed, PBRP2 expression is restricted to reproductive organs and seeds as shown by RT-PCR, immunofluorescence labelling and GUS staining experiments. Interestingly, pbrp2 loss-of-function specifically affects the development of the syncytial endosperm, with both parental contributions required for wild-type development. pBRP2, is the first B-factor to exhibit cell-specific expression and regulation in eukaryotes, and might play a role in enforcing bi-parental reproduction in angiosperms.



Autor: Emilie Cavel, Marion Pillot, Dominique Pontier, Sylvie Lahmy, Natacha Bies-Etheve, Danielle Vega, Daniel Grimanelli , Thierry Lag

Fuente: http://plos.srce.hr/



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