A Recombinant Vaccine of H5N1 HA1 Fused with Foldon and Human IgG Fc Induced Complete Cross-Clade Protection against Divergent H5N1 VirusesReportar como inadecuado




A Recombinant Vaccine of H5N1 HA1 Fused with Foldon and Human IgG Fc Induced Complete Cross-Clade Protection against Divergent H5N1 Viruses - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Development of effective vaccines to prevent influenza, particularly highly pathogenic avian influenza HPAI caused by influenza A virus IAV subtype H5N1, is a challenging goal. In this study, we designed and constructed two recombinant influenza vaccine candidates by fusing hemagglutinin 1 HA1 fragment of A-Anhui-1-2005H5N1 to either Fc of human IgG HA1-Fc or foldon plus Fc HA1-Fdc, and evaluated their immune responses and cross-protection against divergent strains of H5N1 virus. Results showed that these two recombinant vaccines induced strong immune responses in the vaccinated mice, which specifically reacted with HA1 proteins and an inactivated heterologous H5N1 virus. Both proteins were able to cross-neutralize infections by one homologous strain clade 2.3 and four heterologous strains belonging to clades 0, 1, and 2.2 of H5N1 pseudoviruses as well as three heterologous strains clades 0, 1, and 2.3.4 of H5N1 live virus. Importantly, immunization with these two vaccine candidates, especially HA1-Fdc, provided complete cross-clade protection against high-dose lethal challenge of different strains of H5N1 virus covering clade 0, 1, and 2.3.4 in the tested mouse model. This study suggests that the recombinant fusion proteins, particularly HA1-Fdc, could be developed into an efficacious universal H5N1 influenza vaccine, providing cross-protection against infections by divergent strains of highly pathogenic H5N1 virus.



Autor: Lanying Du, Virtual Ho-Chuen Leung, Xiujuan Zhang, Jie Zhou, Min Chen, Wu He, Hai-Ying Zhang, Chris C. S. Chan, Vincent Kwok-Man

Fuente: http://plos.srce.hr/



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