Double-Positive CD21 CD27 B Cells Are Highly Proliferating Memory Cells and Their Distribution Differs in Mucosal and Peripheral TissuesReportar como inadecuado




Double-Positive CD21 CD27 B Cells Are Highly Proliferating Memory Cells and Their Distribution Differs in Mucosal and Peripheral Tissues - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

Several B-cell defects arise in HIV infected patients, particularly in patients with chronic infection and high viral load. Loss of memory B cells CD27+ B cells in peripheral blood and lymphoid tissues is one of the major B cell dysfunctions in HIV and simian immunodeficiency virus SIV infection. Despite several studies, definitive identification of memory B cells based on CD27 surface expression has not been described. Similarly, the rates of cell turnover in different B cell subpopulation from lymphoid and mucosal tissues have not been well documented. In this study, we demonstrate the presence of memory B cell populations and define their distribution, frequency and immunophenotype with regards to activation, proliferation, maturation, and antibody production in normal rhesus macaques from different lymphoid tissues.

Methodology-Principal Findings

Thirteen healthy, uninfected rhesus macaques were selected for this study. CD20+ B cells were isolated from peripheral blood and sorted based on CD27 and CD21 surface markers to define memory B cell population. All the B cell subpopulation was further characterized phenotypically and their cell turnover rates were evaluated in vivo following bromodeoxyuridine BrdU inoculation. Double positive DP CD21+CD27+ B cells in both peripheral and lymphoid tissues are memory B cells, able to produce antibody by polyclonal activation, and without T cell help. Peripheral and lymphoid DP CD21+CD27+ B cells were also able to become activated and proliferate at higher rates than other B cell subpopulations. Increased turnover of tonsillar memory B cells were identified compared to other tissues examined.

Conclusions-Significance

We suggest that this DP memory B cells play a major role in the immune system and their function and proliferation might have an important role in HIV-SIV mediated B cell dysregulation and pathogenesis.



Autor: Arpita Das, Huanbin Xu, Xiaolei Wang, Canddy L. Yau, Ronald S. Veazey, Bapi Pahar

Fuente: http://plos.srce.hr/



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