High-Efficient Generation of Induced Pluripotent Stem Cells from Human AstrocytesReportar como inadecuado




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The reprogramming of human somatic cells to induced pluripotent stem hiPS cells enables the possibility of generating patient-specific autologous cells for regenerative medicine. A number of human somatic cell types have been reported to generate hiPS cells, including fibroblasts, keratinocytes and peripheral blood cells, with variable reprogramming efficiencies and kinetics. Here, we show that human astrocytes can also be reprogrammed into hiPS ASThiPS cells, with similar efficiencies to keratinocytes, which are currently reported to have one of the highest somatic reprogramming efficiencies. ASThiPS lines were indistinguishable from human embryonic stem ES cells based on the expression of pluripotent markers and the ability to differentiate into the three embryonic germ layers in vitro by embryoid body generation and in vivo by teratoma formation after injection into immunodeficient mice. Our data demonstrates that a human differentiated neural cell type can be reprogrammed to pluripotency and is consistent with the universality of the somatic reprogramming procedure.



Autor: Sergio Ruiz, Kristen Brennand, Athanasia D. Panopoulos, Aída Herrerías, Fred H. Gage, Juan Carlos Izpisua-Belmonte

Fuente: http://plos.srce.hr/



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