The Integrative Analysis of microRNA and mRNA Expression in Mouse Uterus under Delayed Implantation and ActivationReportar como inadecuado




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Background

Delayed implantation is a developmental arrest at the blastocyst stage and a good model for embryo implantation. MicroRNAs miRNAs have been shown to be involved in mouse embryo implantation through regulating uterine gene expression. This study was to have an integrative analysis on global miRNA and mRNA expression in mouse uterus under delayed implantation and activation through Illumina sequencing.

Methodology-Principal Findings

By deep sequencing and analysis, we found that there are 20 miRNAs up-regulated and 42 miRNAs down-regulated at least 1.2 folds, and 268 genes up-regulated and 295 genes down-regulated at least 2 folds under activation compared to delayed implantation, respectively. Many different forms of editing in mature miRNAs are detected. The percentage of editing at positions 4 and 5 of mature miRNAs is significantly higher under delayed implantation than under activation. Although the number of miR-21 reference sequence under activation is slightly lower than that under delayed implantation, the total level of miR-21 under activation is higher than that under delayed implantation. Six novel miRNAs are predicted and confirmed. The target genes of significantly up-regulated miRNAs under activation are significantly enriched.

Conclusions

miRNA and mRNA expression patterns are closely related. The target genes of up-regulated miRNAs are significantly enriched. A high level of editing at positions 4 and 5 of mature miRNAs is detected under delayed implantation than under activation. Our data should be valuable for future study on delayed implantation.



Autor: Ren-Wei Su , Wei Lei , Ji-Long Liu, Zhi-Rong Zhang, Bo Jia, Xu-Hui Feng, Gang Ren, Shi-Jun Hu, Zeng-Ming Yang

Fuente: http://plos.srce.hr/



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