Rat Adipose Tissue-Derived Stem Cells Transplantation Attenuates Cardiac Dysfunction Post Infarction and Biopolymers Enhance Cell RetentionReportar como inadecuado




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Background

Cardiac cell transplantation is compromised by low cell retention and poor graft viability. Here, the effects of co-injecting adipose tissue-derived stem cells ASCs with biopolymers on cell cardiac retention, ventricular morphometry and performance were evaluated in a rat model of myocardial infarction MI.

Methodology-Principal Findings

99mTc-labeled ASCs 1×106 cells isolated from isogenic Lewis rats were injected 24 hours post-MI using fibrin a, collagen ASC-C, or culture medium ASC-M as vehicle, and cell body distribution was assessed 24 hours later by γ-emission counting of harvested organs. ASC-F and ASC-C groups retained significantly more cells in the myocardium than ASC-M 13.8±2.0 and 26.8±2.4% vs. 4.8±0.7%, respectively. Then, morphometric and direct cardiac functional parameters were evaluated 4 weeks post-MI cell injection. Left ventricle LV perimeter and percentage of interstitial collagen in the spare myocardium were significantly attenuated in all ASC-treated groups compared to the non-treated NT and control groups culture medium, fibrin, or collagen alone. Direct hemodynamic assessment under pharmacological stress showed that stroke volume SV and left ventricle end-diastolic pressure were preserved in ASC-treated groups regardless of the vehicle used to deliver ASCs. Stroke work SW, a global index of cardiac function, improved in ASC-M while it normalized when biopolymers were co-injected with ASCs. A positive correlation was observed between cardiac ASCs retention and preservation of SV and improvement in SW post-MI under hemodynamic stress.

Conclusions

We provided direct evidence that intramyocardial injection of ASCs mitigates the negative cardiac remodeling and preserves ventricular function post-MI in rats and these beneficial effects can be further enhanced by administrating co-injection of ASCs with biopolymers.



Autor: Maria E. Danoviz, Juliana S. Nakamuta, Fabio L. N. Marques, Leonardo dos Santos, Erica C. Alvarenga, Alexandra A. dos Santos, Edn

Fuente: http://plos.srce.hr/



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