Different Oxidative Stress Response in Keratinocytes and Fibroblasts of Reconstructed Skin Exposed to Non Extreme Daily-Ultraviolet RadiationReportar como inadecuado




Different Oxidative Stress Response in Keratinocytes and Fibroblasts of Reconstructed Skin Exposed to Non Extreme Daily-Ultraviolet Radiation - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Experiments characterizing the biological effects of sun exposure have usually involved solar simulators. However, they addressed the worst case scenario i.e. zenithal sun, rarely found in common outdoor activities. A non-extreme ultraviolet radiation UV spectrum referred as -daily UV radiation- DUVR with a higher UVA 320–400 nm to UVB 280–320 nm irradiance ratio has therefore been defined. In this study, the biological impact of an acute exposure to low physiological doses of DUVR corresponding to 10 and 20% of the dose received per day in Paris mid-April on a 3 dimensional reconstructed skin model, was analysed. In such conditions, epidermal and dermal morphological alterations could only be detected after the highest dose of DUVR. We then focused on oxidative stress response induced by DUVR, by analyzing the modulation of mRNA level of 24 markers in parallel in fibroblasts and keratinocytes. DUVR significantly modulated mRNA levels of these markers in both cell types. A cell type differential response was noticed: it was faster in fibroblasts, with a majority of inductions and high levels of modulation in contrast to keratinocyte response. Our results thus revealed a higher sensitivity in response to oxidative stress of dermal fibroblasts although located deeper in the skin, giving new insights into the skin biological events occurring in everyday UV exposure.



Autor: Claire Marionnet, Cécile Pierrard, François Lejeune, Juliette Sok, Marie Thomas, Françoise Bernerd

Fuente: http://plos.srce.hr/



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