UCP1 Induction during Recruitment of Brown Adipocytes in White Adipose Tissue Is Dependent on Cyclooxygenase ActivityReportar como inadecuado




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Background

The uncoupling protein 1 UCP1 is a hallmark of brown adipocytes and pivotal for cold- and diet-induced thermogenesis.

Methodology-Principal Findings

Here we report that cyclooxygenase COX activity and prostaglandin E2 PGE2 are crucially involved in induction of UCP1 expression in inguinal white adipocytes, but not in classic interscapular brown adipocytes. Cold-induced expression of UCP1 in inguinal white adipocytes was repressed in COX2 knockout KO mice and by administration of the COX inhibitor indomethacin in wild-type mice. Indomethacin repressed β-adrenergic induction of UCP1 expression in primary inguinal adipocytes. The use of PGE2 receptor antagonists implicated EP4 as a main PGE2 receptor, and injection of the stable PGE2 analog EP3-4 agonist 16,16 dm PGE2 induced UCP1 expression in inguinal white adipose tissue. Inhibition of COX activity attenuated diet-induced UCP1 expression and increased energy efficiency and adipose tissue mass in obesity-resistant mice kept at thermoneutrality.

Conclusions-Significance

Our findings provide evidence that induction of UCP1 expression in white adipose tissue, but not in classic interscapular brown adipose tissue is dependent on cyclooxygenase activity. Our results indicate that cyclooxygenase-dependent induction of UCP1 expression in white adipose tissues is important for diet-induced thermogenesis providing support for a surprising role of COX activity in the control of energy balance and obesity development.



Autor: Lise Madsen , Lone M. Pedersen, Haldis Haukaas Lillefosse, Even Fjære, Ingeborg Bronstad, Qin Hao, Rasmus K. Petersen, Philip Ha

Fuente: http://plos.srce.hr/



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