Studying the formation of aggregates in recombinant human granulocyte-colony stimulating factor rHuG-CSF, lenograstim, using size-exclusion chromatography and SDS-PAGEReportar como inadecuado




Studying the formation of aggregates in recombinant human granulocyte-colony stimulating factor rHuG-CSF, lenograstim, using size-exclusion chromatography and SDS-PAGE - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Acta Pharmaceutica, Vol.58 No.2 June 2008. -

The stability of proteins is a subject of intense current interest. Aggregation, as a dominant degradation pathway for therapeutic proteins, may cause multiple adverse effects, including loss of efficacy and immunogenicity. In the present study, the formation of aggregates in lenograstim under physiological conditions was monitored. For this purpose, a simple and selective size-exclusion high-performance liquid chromatography method for detection and separation of aggregates from intact protein was developed. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis was performed under reducing and non-reducing conditions to determine the nature of aggregate bond formation. Using both techniques, the presence of a low aggregate content attached via disulfide bonds was detected.

lenograstim; aggregate formation; size-exclusion high-performance liquid chromatography; sodium dodecyl sulphate-polyacrylamide gel electrophoresis



Autor: JASMINA TONIC RIBARSKA - orcid.org-0000-0002-0026-3252 ; Faculty of Pharmacy, University -Ss Cyril and Methodius-, 1000 Skopje, M

Fuente: http://hrcak.srce.hr/



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