Evaluation of a Recombinant Measles Virus as the Expression Vector of Hepatitis C Virus Envelope ProteinsReportar como inadecuado




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Measles virus MV is a negative strand RNA virus of the family Paramyxoviridae, and the attenuated Edmonston-B strain can be engineered by the reverse genetics system. Here we constructed the recombinant Edmonston strain of measles virus MV-Ed that expressed hepatitis C virus HCV envelope proteins rMV-E1E2. The rMV-E1E2 successfully expressed HCV E1 and E2 proteins. To evaluate its immunogenicity, NOD-Scid-Jak3null mice that were engrafted with human peripheral blood mononuclear cells huPBMC-NOJ were infected with this rMV-E1E2. Although human lymphocytes could be isolated from the spleens of mock-infected mice during the 2-weeks-long experiment, the proportion of mice that were infected with MV or rMV-E1E2 was decreased in a viral dose-dependent manner. Over 103 PFU of virus infection decreased the human PBL to less than 5%. Significant decrease of B cell population in human PBL from rMV-E1E2 infected NOD-SCID mice and decrease of T cell population in those from MV infected mice were observed. Human antibody production in these mice was also examined. Thus, the results in this study may contribute for future improvement of recombinant vaccine using measles virus vector.

KEYWORDS

MV, HCV, E1, E2, Human PBMC, NOD-Scid-Jak3null Mouse

Cite this paper

Y. Kasama, M. Satoh, M. Saito, S. Okada, C. Kai and K. Tsukiyama-Kohara -Evaluation of a Recombinant Measles Virus as the Expression Vector of Hepatitis C Virus Envelope Proteins,- World Journal of Vaccines, Vol. 1 No. 3, 2011, pp. 98-103. doi: 10.4236-wjv.2011.13010.





Autor: Yuri Kasama, Masaaki Satoh, Makoto Saito, Seiji Okada, Chieko Kai, Kyoko Tsukiyama-Kohara

Fuente: http://www.scirp.org/



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