Involvement of Transcription Factor NR2F2 in Human Trophoblast DifferentiationReportar como inadecuado

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During the in vitro differentiation of human villous cytotrophoblast CTB cells to a syncytiotrophoblast STB phenotype, mRNA levels for the nuclear hormone receptor NR2F2 ARP-1, COUP-TFII increase rapidly, reaching a peak at day 1 of differentiation that is 8.8-fold greater than that in undifferentiated CTB cells. To examine whether NR2F2 is involved in the regulation of villous CTB cell differentiation, studies were performed to determine whether NR2F2 regulates the expression of TFAP2A AP-2α, a transcription factor that is critical for the terminal differentiation of these cells to a STB phenotype.

Methodology-Primary Findings

Overexpression of NR2F2 in primary cultures of human CTB cells and JEG-3 human choriocarcinoma cells induced dose-dependent increases in TFAP2A promoter activity. Conversely, siRNA mediated silencing of the NR2F2 gene in villous CTB undergoing spontaneous differentiation blocked the induction of the mRNAs for TFAP2A and several STB cell specific marker genes, including human placental lactogen hPL, pregnancy specific glycoprotein 1 PSG1 and corticotropin releasing hormone CRH by 51–59%. The induction of TFAP2A promoter activity by NR2F2 was potentiated by the nuclear hormone receptors retinoic acid receptor alpha RARA and retinoid X receptor alpha RXRA.


Taken together, these results strongly suggest that NR2F2 is involved in villous CTB cell differentiation and that NR2F2 acts, at least in part, by directly activating TFAP2A gene expression and by potentiating the transactivation of TFAP2A by RARA and RXRA.

Autor: Michael A. Hubert, Susan L. Sherritt, Cindy J. Bachurski, Stuart Handwerger



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