Determination of Fenofibrate and the Degradation Product Using Simultaneous UV-Derivative Spectrometric Method and HPLCReportar como inadecuado




Determination of Fenofibrate and the Degradation Product Using Simultaneous UV-Derivative Spectrometric Method and HPLC - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Two new selective, precise, and accurate methods were developed for the determination of fenofibrate in the presence of its basic degradation product. In the first method fenofibrate was determined using an algorithm bivariate calibration derivative method, in which an optimum pair of wavelengths was chosen for the determination of different binary mixtures. In the second method HPLC, separation was achieved on RESTEK Pinnacle II phenyl column 5 µm, 250 × 4.6 mm and Pinnacle II phenyl 5 µm, 10 × 4 mm guard cartridge using a mobile phase consisting of methanol –0.1% phosphoric acid 60:40, v-v at a flow rate 2 mL●min–1, and the column oven temperature was set at 50°C. The UV detector was time programmed at 302 nm and 289 nm for the internal standard I.S. and fenofibrate, respectively. The proposed methods were successfully applied for the determination of fenofibrate and its degradation product in the laboratory-prepared mixture and in pharmaceutical formulation. The assay results obtained using the bivariate method were statistically compared to those of the HPLC method and good agreement was observed.

KEYWORDS

Fenofibrate, Stability, Degradation Product, UV Derivative Spectrometric Method, HPLC

Cite this paper

F. Salamaa, M. Nassar, M. El-Din, K. Attia and M. Kaddah -Determination of Fenofibrate and the Degradation Product Using Simultaneous UV-Derivative Spectrometric Method and HPLC,- American Journal of Analytical Chemistry, Vol. 2 No. 3, 2011, pp. 332-343. doi: 10.4236-ajac.2011.23041.





Autor: Fathy M. M. Salamaa, Mohamed W. I. Nassar, Mohie M. K. Sharaf El-Din, Khalid A. M. Attia, Mohamed Yousri Kaddah

Fuente: http://www.scirp.org/



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