How Surface Heterogeneity Affects Protein Adsorption: Annealing of OTS Patterns and Albumin Adsorption KineticsReportar como inadecuado




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Croatica Chemica Acta, Vol.80 No.3-4 November 2007. -

Fluorescence microscopy and intensity histogram analysis techniques were used to monitor

spatially-resolved albumin adsorption kinetics to model heterogeneous surfaces on sub-mm

scales. Several distinct protein subpopulations were resolved, each represented by a normal

distribution of adsorption densities on the adsorbent surface. Histogram analyses provided

dynamic information of mean adsorption density, spread in adsorption density, and surface area

coverage for each distinct protein subpopulation. A simple adsorption model is proposed in

which individual protein binding events are predicted by the summation of multiple protein’s

surface sub-site interactions with different binding energy sub-sites on adsorbent surfaces. This

model is predictive of the albumin adsorption on the patterns produced by one step m-contact

printing mCP of octadecyltrichlorosilane OTS on glass but fails to describe adsorption once

the same patterns are altered by a thermal annealing step.

adsorption kinetics; albumin; surface heterogeneity; fluorescence imaging



Autor: Gerald N. Hodgkinson - ; Department of Bioengineering, University of Utah, Salt Lake City, UT, USA Vladimir Hlady - ; Department

Fuente: http://hrcak.srce.hr/



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