G Protein Coupled Receptors in Embryonic Stem Cells: A Role for Gs-Alpha SignalingReportar como inadecuado




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Background

Identification of receptor mediated signaling pathways in embryonic stem ES cells is needed to facilitate strategies for cell replacement using ES cells. One large receptor family, largely uninvestigated in ES cells, is G protein coupled receptors GPCRs. An important role for these receptors in embryonic development has been described, but little is known about GPCR expression in ES cells.

Methodology-Principal Findings

We have examined the expression profile of 343 different GPCRs in mouse ES cells demonstrating for the first time that a large number of GPCRs are expressed in undifferentiated and differentiating ES cells, and in many cases at high levels. To begin to define a role for GPCR signaling in ES cells, the impact of activating Gs-alpha, one of the major alpha subunits that couples to GPCRs, was investigated. Gs-alpha activation resulted in larger embryoid bodies EBs, due, in part, to increased cell proliferation and prevented the time-related decline in expression of transcription factors important for maintaining ES cell pluripotency.

Significance-Conclusions

These studies suggest that Gs-alpha signaling contributes to ES cell proliferation and pluripotency and provide a framework for further investigation of GPCRs in ES cells.



Autor: Brian T. Layden, Marsha Newman, Fei Chen, Amanda Fisher, William L. Lowe Jr.

Fuente: http://plos.srce.hr/



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