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Somatic embryogenesis was achieved in two oil yielding tropical tree species i.e. Simarouba glauca & Azadirachta indica using immature zygotic embryos as explants on Murashige and Skoog MS medium supplemented with 0.5 – 1.5 mg-l benzylaminopurine BA and 2.0 - 3.0 mg-l NAA 1-napthaleneacetic acid or 2, 4-D 2,4-dichlorophenoxyacetic acid and 3% sucrose. MS medium containing 1.0 mg-l BA and 2.0 mg-l NAA was noted to be the most effective in inducing friable embryogenic callus FEC in Simarouba glauca; the number of somatic embryos per culture varied in MS medium supplemented with 1.0 – 1.5 mg-l BA and 1.0 mg-l NAA. In Azadirachta indica, somatic embryos developed on MS medium supplemented with 0.5 mg-l BA and 1.5 – 2.0 mg-l 2,4-D which were in various shapes and sizes after the first subculture on MS medium supplemented with 0.25 mg-l abscisic acid. The somatic embryos which developed shoots were isolated and rooted in 1-2 strength MS medium supplemented with 0.25 mg-l abscisic acid and 2% sucrose. About 25% of embryos germinated within 20 days of culture in case of Simarouba glauca and 62% in Azadirachta indica. The somatic embryo-derived plantlets were transferred to the field after being hardened in the climate controlled hardening chamber.

KEYWORDS

Somatic Embryogenesis, Immature Zygotic Embryos, Growth Regulators, Oil Yielding Tropical Tree

Cite this paper

P. Das -In Vitro Somatic Embryogenesis in Some Oil Yielding Tropical Tree Species,- American Journal of Plant Sciences, Vol. 2 No. 2, 2011, pp. 217-222. doi: 10.4236-ajps.2011.22023.





Autor: Premananda Das

Fuente: http://www.scirp.org/



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