AGO1 Homeostasis Involves Differential Production of 21-nt and 22-nt miR168 Species by MIR168a and MIR168bReportar como inadecuado




AGO1 Homeostasis Involves Differential Production of 21-nt and 22-nt miR168 Species by MIR168a and MIR168b - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

AGO1 associates with microRNAs miRNAs and regulates mRNAs through cleavage and translational repression. AGO1 homeostasis entails DCL1-dependent production of miR168 from MIR168a and MIR168b transcripts, post-transcriptional stabilization of miR168 by AGO1, and AGO1-catalyzed miR168-guided cleavage of AGO1 mRNA.

Principal Findings

This study reveals that MIR168a is highly expressed and predominantly produces a 21-nt miR168 species. By contrast, MIR168b is expressed at low levels and produces an equal amount of 21- and 22-nt miR168 species. Only the 21-nt miR168 is preferentially stabilized by AGO1, and consequently, the accumulation of the 22-nt but not the 21-nt miR168 is reduced when DCL1 activity is impaired. mir168a mutants with strongly reduced levels of 21-nt miR168 are viable but exhibit developmental defects, particularly during environmentally challenging conditions.

Conclusions-Significance

These results suggest that 22-nt miR168 ensures basal cleavage of AGO1 mRNA whereas 21-nt miR168 permits an effective response to endogenous or environmental fluctuations owing to its preferential stabilization by AGO1.



Autor: Hervé Vaucheret

Fuente: http://plos.srce.hr/



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