Early Low Protein Diet Aggravates Unbalance between Antioxidant Enzymes Leading to Islet DysfunctionReportar como inadecuado




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Background

Islets from adult rat possess weak antioxidant defense leading to unbalance between superoxide dismutase SOD and hydrogen peroxide-inactivating enzymatic activities, catalase CAT and glutathione peroxidase GPX rending them susceptible to oxidative stress. We have shown that this vulnerability is influenced by maternal diet during gestation and lactation.

Methodology-Principal Findings

The present study investigated if low antioxidant activity in islets is already observed at birth and if maternal protein restriction influences the development of islet antioxidant defenses. Rats were fed a control diet C group or a low protein diet during gestation LP or until weaning LPT, after which offspring received the control diet. We found that antioxidant enzymatic activities varied with age. At birth and after weaning, normal islets possessed an efficient GPX activity. However, the antioxidant capacity decreased thereafter increasing the potential vulnerability to oxidative stress. Maternal protein malnutrition changed the antioxidant enzymatic activities in islets of the progeny. At 3 months, SOD activity was increased in LP and LPT islets with no concomitant activation of CAT and GPX. This unbalance could lead to higher hydrogen peroxide production, which may concur to oxidative stress causing defective insulin gene expression due to modification of critical factors that modulate the insulin promoter. We found indeed that insulin mRNA level was reduced in both groups of malnourished offspring compared to controls. Analyzing the expression of such critical factors, we found that c-Myc expression was strongly increased in islets from both protein-restricted groups compared to controls.

Conclusion and Significance

Modification in antioxidant activity by maternal low protein diet could predispose to pancreatic islet dysfunction later in life and provide new insights to define a molecular mechanism responsible for intrauterine programming of endocrine pancreas.



Autor: Nicolas Theys, André Clippe, Thomas Bouckenooghe, Brigitte Reusens, Claude Remacle

Fuente: http://plos.srce.hr/



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