Odorant-Dependent Generation of Nitric Oxide in Mammalian Olfactory Sensory NeuronsReportar como inadecuado

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The gaseous signalling molecule nitric oxide NO is involved in various physiological processes including regulation of blood pressure, immunocytotoxicity and neurotransmission. In the mammalian olfactory bulb OB, NO plays a role in the formation of olfactory memory evoked by pheromones as well as conventional odorants. While NO generated by the neuronal isoform of NO synthase nNOS regulates neurogenesis in the olfactory epithelium, NO has not been implicated in olfactory signal transduction. We now show the expression and function of the endothelial isoform of NO synthase eNOS in mature olfactory sensory neurons OSNs of adult mice. Using NO-sensitive micro electrodes, we show that stimulation liberates NO from isolated wild-type OSNs, but not from OSNs of eNOS deficient mice. Integrated electrophysiological recordings electro-olfactograms or EOGs from the olfactory epithelium of these mice show that NO plays a significant role in modulating adaptation. Evidence for the presence of eNOS in mature mammalian OSNs and its involvement in odorant adaptation implicates NO as an important new element involved in olfactory signal transduction. As a diffusible messenger, NO could also have additional functions related to cross adaptation, regeneration, and maintenance of MOE homeostasis.

Autor: Daniela Brunert, Stefan Kurtenbach, Sonnur Isik, Heike Benecke, Günter Gisselmann, Wolfgang Schuhmann, Hanns Hatt, Christian H.

Fuente: http://plos.srce.hr/


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