Maternal Protein Restriction Affects Postnatal Growth and the Expression of Key Proteins Involved in Lifespan Regulation in MiceReportar como inadecuado




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We previously reported that maternal protein restriction in rodents influenced the rate of growth in early life and ultimately affected longevity. Low birth weight caused by maternal protein restriction followed by catch-up growth recuperated animals was associated with shortened lifespan whereas protein restriction and slow growth during lactation postnatal low protein: PLP animals increased lifespan. We aim to explore the mechanistic basis by which these differences arise. Here we investigated effects of maternal diet on organ growth, metabolic parameters and the expression of insulin-IGF1 signalling proteins and Sirt1 in muscle of male mice at weaning. PLP mice which experienced protein restriction during lactation had lower fasting glucose P = 0.038 and insulin levels P = 0.046 suggesting improved insulin sensitivity. PLP mice had higher relative weights adjusted by body weight of brain P = 0.0002 and thymus P = 0.031 compared to controls suggesting that enhanced functional capacity of these two tissues is beneficial to longevity. They also had increased expression of insulin receptor substrate 1 P = 0.021 and protein kinase C zeta P = 0.046. Recuperated animals expressed decreased levels of many insulin signalling proteins including PI3 kinase subunits p85α P = 0.018, p110β P = 0.048 and protein kinase C zeta P = 0.006 which may predispose these animals to insulin resistance. Sirt1 protein expression was reduced in recuperated offspring. These observations suggest that maternal protein restriction can affect major metabolic pathways implicated in regulation of lifespan at a young age which may explain the impact of maternal diet on longevity.



Autor: Jian-Hua Chen , Malgorzata S. Martin-Gronert, Jane Tarry-Adkins, Susan E. Ozanne

Fuente: http://plos.srce.hr/



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