Inter-MAR Association Contributes to Transcriptionally Active Looping Events in Human β-globin Gene ClusterReportar como inadecuado




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Matrix attachment regions MARs are important in chromatin organization and gene regulation. Although it is known that there are a number of MAR elements in the β-globin gene cluster, it is unclear that how these MAR elements are involved in regulating β-globin genes expression. Here, we report the identification of a new MAR element at the LCRlocus control region of human β-globin gene cluster and the detection of the inter-MAR association within the β-globin gene cluster. Also, we demonstrate that SATB1, a protein factor that has been implicated in the formation of network like higher order chromatin structures at some gene loci, takes part in β-globin specific inter-MAR association through binding the specific MARs. Knocking down of SATB1 obviously reduces the binding of SATB1 to the MARs and diminishes the frequency of the inter-MAR association. As a result, the ACH establishment and the α-like globin genes and β-like globin genes expressions are affected either. In summary, our results suggest that SATB1 is a regulatory factor of hemoglobin genes, especially the early differentiation genes at least through affecting the higher order chromatin structure.



Autor: Li Wang , Li-Jun Di , Xiang Lv, Wei Zheng, Zheng Xue, Zhi-Chen Guo, De-Pei Liu , Chi-Chuan Liang

Fuente: http://plos.srce.hr/



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