Evidence for the Mitochondrial Lactate Oxidation Complex in Rat Neurons: Demonstration of an Essential Component of Brain Lactate ShuttlesReportar como inadecuado




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To evaluate the presence of components of a putative Intracellular Lactate Shuttle ILS in neurons, we attempted to determine if monocarboxylate e.g. lactate transporter isoforms MCT1 and -2 and lactate dehydrogenase LDH are coexpressed in neuronal mitochondria of rat brains. Immunohistochemical analyses of rat brain cross-sections showed MCT1, MCT2, and LDH to colocalize with the mitochondrial inner membrane marker cytochrome oxidase COX in cortical, hippocampal, and thalamic neurons. Immunoblotting after immunoprecipitation IP of mitochondria from brain homogenates supported the histochemical observations by demonstrating that COX coprecipitated MCT1, MCT2, and LDH. Additionally, using primary cultures from rat cortex and hippocampus as well as immunohistochemistry and immunocoprecipitation techniques, we demonstrated that MCT2 and LDH are coexpressed in mitochondria of cultured neurons. These findings can be interpreted to mean that, as in skeletal muscle, neurons contain a mitochondrial lactate oxidation complex mLOC that has the potential to facilitate both intracellular and cell-cell lactate shuttles in brain.



Autor: Takeshi Hashimoto, Rajaa Hussien, Hyung-Sook Cho, Daniela Kaufer, George A. Brooks

Fuente: http://plos.srce.hr/



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