Formin 1-Isoform IV Deficient Cells Exhibit Defects in Cell Spreading and Focal Adhesion FormationReportar como inadecuado




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Background

Regulation of the cytoskeleton is a central feature of cell migration. The formin family of proteins controls the rate of actin nucleation at its barbed end. Thus, formins are predicted to contribute to several important cell processes such as locomotion, membrane ruffling, vesicle endocytosis, and stress fiber formation and disassociation.

Methodology-Principal Findings

In this study we investigated the functional role of Formin1-isoform4 Fmn1-IV by using genetically null primary cells that displayed augmented protrusive behaviour during wound healing and delayed cell spreading. Cells deficient of Fmn1-IV also showed reduced efficiency of focal adhesion formation. Additionally, we generated an enhanced green fluorescence protein EGFP-fused Fmn1-IV knock-in mouse to monitor the endogenous subcellular localization of Fmn1-IV. Its localization was found within the cytoplasm and along microtubules, yet it was largely excluded from adherens junctions.

Conclusions-Significance

It was determined that Fmn1-IV, as an actin nucleator, contributes to protrusion of the cell-s leading edge and focal adhesion formation, thus contributing to cell motility.



Autor: Markus Dettenhofer , Fen Zhou, Philip Leder

Fuente: http://plos.srce.hr/



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