Single Bead Affinity Detection SINBAD for the Analysis of Protein-Protein InteractionsReportar como inadecuado




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We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots QDs, are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.



Autor: Roberta Schulte, Jessica Talamas, Christine Doucet, Martin W. Hetzer

Fuente: http://plos.srce.hr/



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