Novel Peptide Sequence -IQ-tag- with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo ImagingReportar como inadecuado




Novel Peptide Sequence -IQ-tag- with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

Probes that allow site-specific protein labeling have become critical tools for visualizing biological processes.

Methods

Here we used phage display to identify a novel peptide sequence with nanomolar affinity for near infrared NIR benzindolium fluorochromes. The developed peptide sequence -IQ-tag- allows detection of NIR dyes in a wide range of assays including ELISA, flow cytometry, high throughput screens, microscopy, and optical in vivo imaging.

Significance

The described method is expected to have broad utility in numerous applications, namely site-specific protein imaging, target identification, cell tracking, and drug development.



Autor: Kimberly A. Kelly , Jonathan Carson , Jason R. McCarthy, Ralph Weissleder

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados