Evaluation of the Rodent Hershberger Bioassay Using Three Reference (Anti)AndrogensReportar como inadecuado

Evaluation of the Rodent Hershberger Bioassay Using Three Reference (Anti)Androgens - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Archives of Industrial Hygiene and Toxicology, Vol.56 No.2 May 2005. -

Under the umbrella of the Organization for Economic Cooperation and Development (OECD) the rodent Hershberger bioassay is being validated as an in vivo screen for the detection of (anti)androgens. As part of the validation work we studied trenbolone (TREN), 1,1-bis-(4-chlorophenyl)-2,2-dichloroethylene (p,p-DDE) and vinclozolin (VIN). Oral intubation of castrated rats with TREN 0.3-1.5-8-40 mg kg-1 body weight (b.w.) for ten days increased androgen-sensitive tissue weights (ASTW) at the high dose. p,p-DDE (5-16-50-160 mg kg-1 b.w.) and VIN (0-3-10-30-100 mg kg-1 b.w.) orally administered for ten days produced a dose-dependent decrease in ASTW in castrated rats subcutaneously supplemented with testosterone propionate (0.4 mg kg-1 b.w.). p,p-DDE also strongly increased liver weights and induced hepatocellular hypertrophy and thyroid follicular cell hypertrophy that was most likely mediated by liver enzyme induction. Our data strongly suggest that the OECD protocol of the rodent Hershberger bioassay describes a sensitive in vivo screen, capable of detecting weakly active (anti)androgens. Furthermore, our data may also indicate that thyroid effects could be assessed, if the protocol is amended accordingly.

in vivo screen; OECD; p,p-DDE; thyroid; trenbolone; vinclozolin

Autor: Alexius Freyberger - Elke Hartmann - Franc Krötlinger -

Fuente: http://hrcak.srce.hr/


Documentos relacionados