Comprehensive Characterization of Serum MicroRNA Profile in Response to the Emerging Avian Influenza A H7N9 Virus Infection in HumansReportar como inadecuado




Comprehensive Characterization of Serum MicroRNA Profile in Response to the Emerging Avian Influenza A H7N9 Virus Infection in Humans - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Institute of Pathogenic Microbiology, Jiangsu Provincial Center for Disease Control and Prevention, Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health, Nanjing 210009, China

2

Department of Acute Infectious Diseases Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China



These authors contributed equally to this work.





*

Authors to whom correspondence should be addressed.



Abstract A novel avian-origin influenza A H7N9 virus recently occurred in China and caused 137 human infection cases with a 32.8% mortality rate. Although various detection procedures have been developed, the pathogenesis of this emerging virus in humans remains largely unknown. In this study, we characterized serum microRNA miRNA profile in response to H7N9 virus infection using TaqMan Low Density Arrays. Upon infection, a total of 395 miRNAs were expressed in the serum pool of patients, far beyond the 221 in healthy controls. Among the 187 commonly expressed miRNAs, 146 were up-regulated and only 7 down-regulated in patients. Further analysis by quantitative RT-PCR revealed that the serum levels of miR-17, miR-20a, miR-106a and miR-376c were significantly elevated in patients compared with healthy individuals p < 0.05. Receiver operating characteristic ROC curves were constructed to show that each miRNA could discriminate H7N9 patients from controls with area under the curve AUC values ranging from 0.622 to 0.898, whereas a combination of miR-17, miR-20a, miR-106a and miR-376c obtained a higher discriminating ability with an AUC value of 0.96. Our findings unravel the significant alterations in serum miRNA expression following virus infection and manifest great potential of circulating miRNAs for the diagnosis of viral diseases. View Full-Text

Keywords: H7N9 virus; avian influenza; serum microRNA; expression profile H7N9 virus; avian influenza; serum microRNA; expression profile





Autor: Zheng Zhu 1,†, Yuhua Qi 1,†, Aihua Ge 2,†, Yefei Zhu 2, Ke Xu 2, Hong Ji 2, Zhiyang Shi 1, Lunbiao Cui 1,* and Minghao Zhou 1,*

Fuente: http://mdpi.com/



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