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1

Department of Biochemistry, Chang Gung University, Kweishan, Taoyuan 333, Taiwan

2

Department of Cosmetic Science, Chang Gung University of Science and Technology, Kweishan, Taoyuan 333, Taiwan

3

Research Center for Emerging Viral Infections, Chang Gung University, Kweishan, Taoyuan 333, Taiwan

4

Department of Medical Research, Chang Gung Memorial Hospital, Kweishan, Taoyuan 333, Taiwan



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Abstract Bai Shao BS, the root of Paeonia lactiflora Pall., a common Chinese herb in many recipes used to treat viral infection and liver diseases, is recognized for its ability to nourish menstruation, its Yin convergence, and as an antiperspirant. However, the mechanism and components for its antiviral function remain to be elucidated. In this study, an ethanolic extract of BS was further partitioned into aqueous and organic parts EAex for in vitro functional study and in vivo efficacy testing. EAex exhibited an IC50 of 0.016 ± 0.005 mg-mL against influenza virus A-WSN-33 H1N1, with broad-spectrum inhibitory activity against different strains of human influenza A viruses, including clinical oseltamivir-resistant isolates and an H1N1pdm strain. The synthesis of both viral RNA and protein was profoundly inhibited when the cells were treated with EAex. A time-of-addition assay demonstrated that EAex exerted its antiviral activity at various stages of the virus replication cycle. We addressed its antiviral activity at virus entry and demonstrated that EAex inhibits viral hemagglutination and viral binding to and penetration into host cells. In vivo animal testing showed that 200 mg-kg-d of EAex offered significant protection against viral infection. We conclude that BS possesses antiviral activity and has the potential for development as an anti-influenza agent. View Full-Text

Keywords: antiviral; Bai Shao; herbaceous peony; influenza virus; Paeonia lactiflora Pall antiviral; Bai Shao; herbaceous peony; influenza virus; Paeonia lactiflora Pall





Autor: Jin-Yuan Ho 1,†, Hui-Wen Chang 1,†, Chwan-Fwu Lin 2,†, Chien-Jou Liu 1, Chung-Fan Hsieh 1 and Jim-Tong Horng 1,3,4,*

Fuente: http://mdpi.com/



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