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Biodiversity and Biotechnology Research Unit, Biodiversity and Environmental Research Center-BERC, Til, Nablus 970, Palestine



These authors contributed equally to this work.





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Abstract The incidence of watermelon chlorotic stunt disease and molecular characterization of the Palestinian isolate of Watermelon chlorotic stunt virus WmCSV-PAL are described in this study. Symptomatic leaf samples obtained from watermelon Citrullus lanatus Thunb., and cucumber Cucumis sativus L. plants were tested for WmCSV-PAL infection by polymerase chain reaction PCR and Rolling Circle Amplification RCA. Disease incidence ranged between 25%–98% in watermelon fields in the studied area, 77% of leaf samples collected from Jenin were found to be mixed infected with WmCSV-PAL and SLCV. The full-length DNA-A and DNA-B genomes of WmCSV-PAL were amplified and sequenced, and the sequences were deposited in the GenBank. Sequence analysis of virus genomes showed that DNA-A and DNA-B had 97.6%–99.42% and 93.16%–98.26% nucleotide identity with other virus isolates in the region, respectively. Sequence analysis also revealed that the Palestinian isolate of WmCSV shared the highest nucleotide identity with an isolate from Israel suggesting that the virus was introduced to Palestine from Israel. View Full-Text

Keywords: WmCSV; SLCV; Begomovirus; watermelon; Palestine; RCA WmCSV; SLCV; Begomovirus; watermelon; Palestine; RCA





Autor: Mohammed S. Ali-Shtayeh †,* , Rana M. Jamous †, Omar B. Mallah and Salam Y. Abu-Zeitoun

Fuente: http://mdpi.com/



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